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Cd11c

Obesity-associated increases in adipose tissue (AT) CD11c+ cells suggest that dendritic cells (DC), which are involved in the tissue recruitment and activation of macrophages, may play a role in determining AT and liver immunophenotype in obesity. This study addressed this hypothesis. With the use of flow cytometry, electron microscopy, and loss-and-gain of function approaches, the. CD11b is the integrin alpha M chain, CD11c the integrin alpha X chain. CD11b is important for the adherence of neutrophils and monocytes to stimulated endothelium, it also plays a role in.

Cd11c Expression - Start from $48 Each Onl

CD11c-DTR and CD169-DTR are two DTR transgenic mouse strains that deplete CD169 + macrophages (47, 48). Although the CD11c-DTR model mainly depletes cells with high expression of CD11c, thus DCs, it does not spare macrophages that express low levels of this DC marker CD11c (also known as Leu-M5 or Integrin alpha X) [5D11] is a member of the leukointegrin family. CD11c is a cell surface adhesion receptor and is predominantly expressed in tissue macrophages, dendritic cells, monocytes, NK cells and granulocytes. CD11c has been shown to be both sensitive and specific for hairy cell leukemia (HCL) (1). CD11c can be used to differentiate hairy cell leukemia. CD11c is a type I transmembrane protein that is expressed on monocytes, granulocytes, a subset of B cells, dendritic cells, and macrophages and may be demonstrated on B-cell chronic lymphocytic leukemia, marginal zone lymphomas, and hairy cell leukemia. The amount of this antigen is varied with the antigen having dim to partial expression on chronic lymphocytic leukemia and a bright expression on hairy cell leukemia. CD11c is abundantly expressed in monocytes and macrophages. Paraffin reactive antibodies have been developed for this antigen and can certainly be used to give evidence for a histiocytic origin for lesion but make an excellent marker for hairy cell leukemia in paraffin-embedded tissue. Differences between paraffin reactivity and demonstration of this antigen by flow cytometry are noted in that marginal zone lymphomas may express CD11c by flow cytometry, but initial reports are not detected in formalin-fixed, paraffin-embedded material on the same lymphomas.

CD11c (integrin αX subunit, also known as leucocyte surface antigen p150, Axb2 and CR4) is a member of the integrin family. Like other leucocyte integrins (CD11a, CD11b, CD11d), CD11c is non-covalently associated with the β2 integrin subunit (CD18). CD11c is a transmembrane glycoprotein of 145-150 kDa, mainly expressed by monocytes, macrophages and NK cells, and to a lesser extent by. ITGAM (Integrin Subunit Alpha M) is a Protein Coding gene. Diseases associated with ITGAM include Systemic Lupus Erythematosus 6 and Systemic Lupus Erythematosus.Among its related pathways are Innate Immune System and Hematopoietic cell lineage.Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity

Integrin alpha X - Wikipedi

CD11b and CD11c - ResearchGat

  1. Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis
  2. istration of a methionine-choline-deficient diet [36] or nonalcoholic steatohepatitis [37]. Thus, conditional ablation of CD11c+ cells was associated with delayed regression of fibrosis and reduced clearance of activated HSCs, whereas pharmacological or exogenous amplification of the dendritic cell pool accelerated fibrosis recovery. The fibrolytic effect of dendritic cell was correlated in part to the release of MMP-9, a gelatinase that cleaves collagen IV and elastin [36]. Detailed comparison of the respective contribution of dendritic cells and macrophages to fibrolysis will require better phenotypic discri
  3. CD11c (integrin αX subunit, also known as leucocyte surface antigen p150, Axb2 and CR4) is a member of the integrin family. Like other leucocyte integrins (CD11a, CD11b, CD11d), CD11c is non-covalently associated with the β2 integrin subunit (CD18)
  4. Th1 immunity may be particularly limited in the neonate and young infant compared to older individuals because of decreased IL-12 p70 production by CD11c+ DCs, e.g., in response to LPS plus IFN-γ.1 However, this limitation may be pathogen-dependent, as decreased IL-12 production by cord blood CD11c+ DCs is probably comparable to adult CD11c+ DCs after stimulation with certain Gram-positive and -negative bacteria or meningococcal outer-membrane proteins.1 Circulating DCs from cord blood can also allogeneically stimulate cord blood T cells in vitro,1 but it is unclear if they are as effective as adult DCs in promoting Th1 differentiation in an allogeneic context.
  5. CD11c is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.

The paradoxical coexistence of excessive autoantibodies and inadequate affinity-matured pathogen-specific antibodies in lupus is poorly understood. Here, we found that excessive CD11c+T-bet+ age-associated B cells (ABCs)—a recently described autoimmunity-relevant B cell subset [K. Rubtsova et al. , J. Clin. Invest. 127, 1392-1404 (2017)]—not only contribute to the production of. In molecular biology, CD18 (Integrin beta chain-2) is an integrin beta chain protein that is encoded by the ITGB2 gene in humans.Upon binding with one of a number of alpha chains, CD18 is capable of forming multiple heterodimers, which play significant roles in cellular adhesion and cell surface signaling, as well as important roles in immune responses APC/Fire™ 750 anti-mouse CD11c Antibody N418. FC RUO . 117351 . 25 µg $100. 117352 . 100 µg $275. Biotin anti-mouse CD11c Antibody N418. FC, IF RUO . 117303 . 50 µg. CR4 (CD11c/CD18 or p150,95), the third member of the leukocyte β2-integrins, is closely related to CR3. The CR4 α chain (CD11c, 150 kDa) is ∼87% homologous in sequence to CD11b, and recognizes similar ligands including iC3b, fibrinogen and, according to some reports, even ICAM-1. CR4 is expressed preferentially on tissue macrophages that bear only small amounts of CR1 and CR3. Phagocyte CR3 and CR4 differ in cytoplasmic domain structure and attachment to the actin cytoskeleton, and this is thought to provide a mechanism for differential regulation of cellular adhesion or phagocytosis. The relative importance of CR4 as a receptor for iC3b is unclear, as most of the iC3b receptor function of leukocytes, including tissue macrophages, is blocked by use of antibodies to CR3 alone.

CD11c - an overview ScienceDirect Topic

Video: CD11c definition of CD11c by Medical dictionar

CD11c is a member of the leukocyte integrin family of adhesion proteins. It is reported to be expressed in normal tissues, mainly on myeloid cells, for example, in bone marrow myelocytes, premyelocytes, metamyelocytes, non-segmented and segmented neutrophils with high levels reported on tissue macrophages and monocytes and with lowest levels in granulocytes. It is also reported to be expressed on NK cells, activated T cells, lymphoid cell lines, including hairy cell leukemias and a proportion of interdigitating dendritic cells. These CD11c + B220 + Gr1 + cells exhibit an immature plasmacytoid morphology and have been shown to be the major producers of type I IFN in the mouse.10-12 Based on these criteria, CD11c + B220 + Gr1 + cells are presumed to be the murine equivalent of human PDCs With unique expertise across the patient journey from tissue acquisition to treatment, Leica Biosystems is focused on driving innovations by connecting people across radiology, pathology, surgery and oncology - leading and breakthrough outcomes for you and your patients.Blood derived CD11c− and CD11c+ DCs, as well as monocyte-derived DCs, synthesize IL-8, and TNF alpha in response to influenza (0.05–2 ng/ml/104 cells, (Cella et al., 1999a)). As described above, the CD11c− subset is specialized to produce significantly greater quantities of IFN alpha than their monocyte-derived counterparts. Monocyte-derived DCs, but not the lymphoid derived CD11c− DCs also produce IL-15, an important factor for the activation and differentiation of CD8+ T cells (Ma et al., 2000). While the CD11c− subset skews T cell responses towards the TH2 phenotype, monocyte-derived DCs skew responses towards the TH1 phenotype by virtue of their ability to produce IL-12 (Cella et al., 1999a, 1999b). Theoretically, the outcome of influenza infection could be substantially altered by the type of DCs that are infected. novel CD11c+ B-cell subset has been identified that is critical for the development of autoimmunity. However, the role of CD11c+ B cells in the development of lupus is unclear. Chronic graft-versus-host disease (cGVHD) is a lupus-like syndrome with high autoantibody production. The purpose of this study was to explore the role of CD11c+ B cells i

THE EFFECT OF TISSUE MICROENVIRONMENTS ON DC EFFECTORS

) and Cd11c-cre.Irf4 fl/WT progeny were backcrossed to IRF4 fl/fl mice. Genotypic analysis of Cre + offspring showed that many of these mice were Cd11c-cre. Irf4 fl/−, demonstrating that Cre recombinase expression under the CD11c promoter could induce deletion of the Irf4 fl allele in germline. We thus backcrossed Cd11c-cre.Irf4 fl/− or Cd11c-cre.Irf4 fl/fl animals with Irf4 fl/fl mice. All content on this website, including dictionary, thesaurus, literature, geography, and other reference data is for informational purposes only. This information should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional. CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a gene that encodes for CD11c. CD11c is an integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain Blood CD11c(+) DCs expressed similar levels of HLA-DR, CD40, CD86, and CD83 as Mo-DCs. CD209 was present on Mo-DCs but not on blood CD11c(+) DCs. Blood CD11c(+) DCs generated a lower proliferative mixed leukocyte response (MLR) than Mo-DCs

A characteristic subset of microglia expressing CD11c appears in response to brain damage. However, the functional role of CD11c + microglia, as well as the mechanism of its induction, are poorly understood. Here we report that the genetic ablation of signal regulatory protein α (SIRPα), a membrane protein, induced the emergence of CD11c + microglia in the brain white matter CD11c is a type I transmembrane protien found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis

Besides, CD11c is frequently expressed by PLL 38 and MZL subtypes 3, 39, and this marker could be valuable to separate MCL from CD5+ MZL and PLL cases. PLL is a rare and aggressive MBN, characterized by splenomegaly, marked lymphocytosis, and >55% prolymphocytes on PB T1 - Increased TREM-2 expression on the subsets of CD11c+ cells in the lungs and lymph nodes during allergic airway inflammation. AU - Hall, Sannette C. AU - Agrawal, Devendra K. PY - 2017/12/1. Y1 - 2017/12/

Animal models and treatments. Female, CD11c DTR 28 and CD206 DTR mice 26,27, from 4 to-12-weeks-old, excluding 6 to 8 week-old mice, in which ovulatory number is unstable due to the effect of. CD11c c F CD45 I eosinophils CD4+ CD8+ c CD19 r CD3 CD4 CD8 TCRβ gd R C) Gated on DAPI-CD45+ gdT cells NKT B) CD45 I 7 CD90.2 ST2 a-1 ILC2 Figure 1S. Representative flow cytometry gating scheme for lung and spleen granulocytes, ILC2s, and lymphocytes in the lung and spleen.C57BL/6 mice were treated with PBS, IL-33, PBS+S. aureus, or IL-33+S.

Dendritic cells are a type of antigen-presenting cell (APC) that form an important role in the adaptive immune system. The main function of dendritic cells is to present antigens and the cells are. In normal cells, CD11c is expressed on activated CD4/CD8+ T cells, granulocytes, lymphocytes, macrophages, and NK cells. In diseased, cells, CD11c is detected on acute myeloid leukemia (AML)-M4 and M5, hairy cell leukemia, lymphoplasmacytic lymphoma (81%), small lymphocytic lymphoma (SLL), splenic lymphoma, Langerhans cell histiocytosis, sinus histiocytosis, psoriatic skin lesions, and some.

CD11b: a type I transmembrane protein found on monocytes, macrophages, granulocytes, some B cells, dendritic cells, and natural killer cells that facilitates cell adhesion, phagocytosis, and/or chemotaxis; expressed in some B-cell chronic lymphocytic leukemias, most acute nonlymphocytic leukemias, and some hairy cell leukemias Orthogonal Strategies: Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues.Corresponding ITGAX RNA-seq data are presented for the same tissues

Marginal zone lymphoma should be considered if both CD5 and CD10 are negative; Hairy cell leukemia (HCL) has a characteristic phenotype that is CD5-, CD10-, CD11c+, CD22+, CD25+, and CD103+ CD103 antigen (also known as B-ly7) is present in virtually all cases; CD11c and CD25 are less specific but present in almost all cases of hairy cell leukemi To demonstrate that CD11c+ and/or CD11b+/CD11c+ cells in mouse peripheral blood and spleens are primarily DC and their myeloid progenitors, we assessed the adhesion molecule expression in CD3+ T cells and CD19+ B cells in peripheral blood and spleens of 3-month-old mice by using three-color flow cytometry analysis CD11c-DTR/GFP transgenic line 57 has the CD11c promoter ( Itgax ) directing expression of a diphtheria toxin receptor - enhanced green fluorescent protein (DTR/EGFP) fusion protein to dendritic cell populations. Administration of diphtheria toxin results in depletion of dendritic cell populations. CD11c-DTR/GFP transgenic mice are useful in studies of mononuclear phagocyte origins and the.

CD11c+ Lymphoid Tissue Dendritic Cells

Mac-1 is a member of the β 2-integrin family of adhesion molecules, which includes LFA-1 (CD11a), Mac-1 (CD11b) and p150,95 (CD11c) . Each of these cell surface molecules is a heterodimer comprising a unique α subunit associated with the common β 2 chain (CD18) CD11c 39 results for CD11c Sort by. Clear all Done. Filters. Product types Clear. Primary antibodies (39) Proteins and Peptides (1) Research areas Clear. Immunology (39) Signal Transduction (39) Stem Cells (39) Target / Protein Clear. CD11c (39) CD11b+CD11c (5). CD11c is a member of the leukocyte specific integrin family, involved in adherence to activated endothelial cells and complement-mediated phagocytosis. CD11c is normally expressed on histiocytes and monocytes, and weakly expressed on granulocytes This is a Validated Antibody Database (VAD) review about human CD11c, based on 168 published articles (read how Labome selects the articles), using CD11c antibody in all methods.It is aimed to help Labome visitors find the most suited CD11c antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are. Wide species reactivity, guaranteed quality. CD11c antibodies for your research. View datasheets

Our CD11c Antibodies can be used in a variety of model species: Canine, Human, Mouse, Porcine, Primate. Use the list below to choose the CD11c Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products Plasmid ANTI-CD11c IMMUNOTOXIN-HIS TAGS from Dr. Jose Conejo-Garcia's lab contains the insert Anti-CD11 Immunotoxin + His Tags and is published in Cancer Res. 2008 Sep 15. 68(18):7684-91. This plasmid is available through Addgene The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow-derived dendritic cells. In spleen and lymph node, CD11c is expressed at high levels on conventional CD11c+ CD45R- mPDCA-1- dendritic cells, and at moderate levels on CD11c+ CD45R.

Circulating and monocyte-derived DCs

(A) Schematic representation of treatments to differentiate THP-1 monocytes (Mo) into macrophages (M0) or polarized macrophages (M1, M2). (B) Morphological and cell surface marker expression (CD11c, CD11b) changes observed by microscopic and flow cytometric analysis of PMA (phorbol 12-myrisate 13-acetate) differentiated M0. FCS: forward-scatter; SSC: side-scatter. (C) Resting CD38- / CD209- M0. Anti-CD11c Antibody (mouse), PE-Cy7, clone N418; Synonym: Integrin alpha-X, CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c; find Sigma-Aldrich-MABF533 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich cd11c 47 results for cd11c Sort by. Clear all Done. Filters. Product types Clear. Primary antibodies (47) Monoclonals (47) More primary antibodies (47) Flow cytometry antibodies (33) Conjugated Primary Antibodies (22) Rabbit Monoclonal Antibodies (7) Research areas. Mice harboring the Itgax-cre (Cd11c-Cre) BAC transgene express Cre recombinase under control of the integrin alpha X gene ( Itgax or Cd11c) promoter/enhancer regions within the BAC transgene. These Cd11c-Cre transgenic mice are a Cre-lox tool for deletion of floxed sequences in CD8 - , CD8 + dendritic cells, tissue-derived dendritic cells from lymph nodes, lung and epidermis, as well as.

CD11c - BioLegen

  1. CD11c: Alternative Names (click to expand) Anti-CD 11c antibody, anti-CD11 antigen like family member C antibody, anti-CD11 antigen-like family member C antibody, anti-CD11c antibody, anti-CD11c antigen antibod
  2. To generate conditional CD11c‐specific Ppard knockout mice, mice which is homozygous for a Ppard allele with loxP sites on either side of exon 4 (Ppard f/f) 20 on the C57BL/6 background were bred with Itgax‐cre (CD11c cre/+) mice on C57BL/6 background. 21 The Ppard f/f mice and Ppard f/f; CD11c Cre/+ mice were then bred with Apoe -/- mice
  3. Dendritic cells (DCs), named for their probing, 'tree-like' or dendritic shapes, are responsible for the initiation of adaptive immune responses and hence function as the 'sentinels' of the immune system. Paul Langerhans first described DCs in human skin in 1868 but thought they were cutaneous nerve cells. DCs are bone marrow (BM)-derived leukocytes and are the most poten
  4. In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted.
  5. Since both mDC and pDC are CD11c positive [14-16], CD11c is commonly used as a murine DC marker along with costimulator molecules like MHC II, CD40, CD80, and CD86 [17]. Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Metho

Description. CD11c is a 150 kD glycoprotein also known as α X integrin, CR4, and p150. CD11c forms a α X β 2 heterodimer with β 2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells Background/Purpose: Recently, a population of CD11c+ age-associated B cells (ABCs) was identified in normal aged female mice. These cells could be expanded following activation by TLR7 agonists and their presence was associated with autoantibody production. The goals of the current study were to determine: a) when ABCs develop during B cell maturation in the spleen; [

CD11c NeoGenomics Laboratorie

  1. CD11c + DCs are protected against HCMV infection by autocrine production of IFN-α, whereas PDCs might use other mechanisms. PDCs (A-C) or CD11c + DCs (D-G) were incubated with mock or TB40/E (MOI, 20) in the absence or presence of IFN-blocking cocktail, as indicated
  2. 151510 - integrin, alpha-x; itgax - leukocyte surface antigen p150,95, alpha subunit;; leu m5, alpha subunit;; myeloid membrane antigen, alpha subunit;; cd11c - itga
  3. Don't have a ? Register today to view list prices and apply for an account to purchase online!
  4. The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet
  5. Hairy cell leukemia is positive for all common B-cell antigens (CD19, CD20, CD79a, FMC-7, and PAX5), with characteristic expression of annexin A1 (Figure 7, A), CD11c, CD25, CD103, CD123, DBA-44 (CD72) (Figure 7, B), the Hector Battifora mesothelial epitope-1 (HBME-1), pERK, T-bet, and TRAP 58-61 but is typically negative for CD5 and CD10.
  6. istration in patients with chronic plaque-type psoriasis

The CD11c + macrophage gating strategy is depicted in Figure E1 in the online supplement. The CD11c + macrophage number was calculated by multiplying the percentage of gated cells measured by flow cytometry by the original hemocytometer count of total cells recovered for each animal CD11c/CD18 is found on monocytes/macrophages, gran-ulocytes, and subsets of dendritic cells (DCs) in humans10,11 and on DCs and subsets of monocytes/macrophages in mice.12,13 CD11c binds a variety of ligands including ICAM-1, ICAM-2, fibrinogen, collagen, iC3b, and lipopoly-saccharide10,11,14 and was recently demonstrated in vitro t CD11c+ B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c+ B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c+ B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if. Results We found that Cd11c-Cre + Irf4 flox/flox mice lack CD103 + CD11b + DCs, a DC subset unique to the intestine whose function is poorly understood. Their absence in the intestinal muscularis reduced pathogenic inducible nitric oxide synthase (iNOS) production by monocytes and macrophages and ameliorated POI

CD11c + cell depletion was achieved with D.tox injections in transgenic mice and AAA formation and growth was assessed. CD11c + cell depletion was shown to reduce the maximum diameter of AAAs induced by 28-days of AngII infusion. CD11c + depletion commencing after AAA establishment by 14 days of AngII infusion was also shown to lead to smaller. CD Markers - CD11c. This website is intended for pathologists and laboratory personnel, who understand that medical information is imperfect and must be interpreted using reasonable medical judgment

An Introduction to Immunohistochemistry. Immunohistochemistry (IHC) is used in histology to detect the presence of specific protein marker that can assist with accurate tumor classification and diagnosis.This guide illustrates the basic steps used to create an IHC stain CD11c + Dendritic Cells Maintain Antigen Processing, Presentation Capabilities, and CD4 + T-Cell Priming Efficacy Under Hypercholesterolemic Conditions Associated With Atherosclerosis. René R.S. Packard, Elena Maganto-García, Israel Gotsman, Ira Tabas, Peter Libby, and ; Andrew H. Lichtman; From the Leducq Center for Cardiovascular Research and Donald W. Reynolds Cardiovascular Clinical.

CD11c + resident macrophages drive hepatocyte death-triggered liver fibrosis in a murine model of nonalcoholic steatohepatitis Michiko Itoh, 1 Takayoshi Suganami, 2,3 Hideaki Kato, 2,4 Sayaka Kanai, 5 Ibuki Shirakawa, 1 Takeru Sakai, 2 Toshihiro Goto, 2 Masahiro Asakawa, 2 Isao Hidaka, 6 Hiroshi Sakugawa, 7 Koji Ohnishi, 8 Yoshihiro Komohara, 8. Mouse anti Dog CD11c antibody, clone CA11.6A1 recognizes the canine CD11c cell surface antigen, a member of the alpha integrin family. Canine CD11c is expressed by monocytes, granulocytes and by dendritic cells. Mouse anti Dog CD11c, clone CA11.6A1 immunoprecipitates proteins of approximately 95 kDa, corresponding to the common β chain of the CD11/CD18 heterodimer and ~150 kDa, the CD11c. DR+lin−CD11c− cells with features of lymphoid DCs have been demonstrated in cord blood cells, where they comprise about 0.3% of the total cell population (Sorg et al., 1999). CD34-negative selection is also used to purify these cells. These cells are lin−CD4+CD11c−CD45RA+, and express IL-3α receptor but not CD80, CD83 or CD86 and are comparable to similar cells circulating in peripheral blood. CD11c+ precursor cells are much rarer. Effects of culture of these cells in cytokines has not yet been reported. CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. Background. LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a. Peripheral Blood CD11c+ CD21- Age-Associated B Cells (ABCs) in Human Systemic Lupus Erythematosus Are Associated with Innate Type III Interferon and Disease Activity Jennifer L. Barnas , Lin Gao, Mary O'Connell, Jennifer Albrecht, Nida Meednu, R. John Looney and Jennifer Anolik, Medicine- Allergy, Immunology and Rheumatology, University of.

CD11c - IHC Primary Antibodies - Leica Biosystem

  1. CD11c Antibodies . CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils
  2. In mice, CD11c is expressed on dendritic cells, macrophages, monocytes, granulocytes, NK cells, and a subset of T cells. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD11c Positive Selection Kit II (Catalog #18780)
  3. CD11c: >113 Mean Fluorescene Intensity (MFI) Interpretive Data. LAD-1 is an autosomal recessive disorder caused by mutations in CD18, the common chain of the beta-2 integrin family. The beta-2 integrin family is comprised of LFA-1 (CD11a/CD18), Mac-1/CR3 (CD11b/CD18) and p150/95 (CD11c/CD18)
  4. Therefore, defects in CD18 expression lead to either very low or no surface membrane expression of CD11a, CD11b, and CD11c. Severe and moderate forms of LAD-1 exist, differing in the degrees of protein deficiency, which are caused by different ITGB2 mutations. Two relatively distinct clinical phenotypes of LAD-1 have been described
  5. Human CD11c+ LT DCs are found in the thymus, spleen, peripheral lymph nodes, and other secondary lymphoid tissues, and in small numbers in the blood. They can be divided into two major subsets: blood DC antigen (BDCA)-1+ and BDCA-3+ CD11c+ DCs. These and other CD11c+ DC populations that are not pDCs are often referred to in the older literature as conventional DCs. The term myeloid DCs, which has also been used, is obsolete and should be avoided, because all DC populations are myeloid derived. CD11c+ LT DC development in the bone marrow requires expression by DC precursors of Flt3, a cytokine receptor, and its binding of FMS-related tyrosine kinase 3 (Flt3)-ligand, which is produced by nonhematopoietic stromal cells.41

Anti-CD11c Antibodies Invitroge

  1. The subset of circulating CD11c+ DCs, which are likely the human equivalent of the murine CD11b+ subset of LT and migratory DCs, basally express high levels of MHC class II and other proteins involved in MHC class II antigen. As activated/mature DCs, they appear to be specialized for initiating immune responses by naïve CD4 T cells or, as immature DCs, for their tolerance induction. BDCA-1+ CD11c+ DCs express a number of receptors for PAMPs, which include TLRs, NLRs, CLRs, and RLRs (see later). PAMP receptor engagement results in their switching from a tolerance program to an activation program that initiates the T cell immune response. The BDCA-1 molecule (CD1c) is involved in nonclassic antigen presentation of mycobacterial products (mycoketides and lipopeptides) to T cells,42 but it is unclear whether CD1c has additional roles in CD11c+ DC function.
  2. Armenian Hamster Monoclonal CD11c antibody [N418]. Validated in ICC/IF, IHC-P, IHC-Fr, FACS, IP. Tested in Mouse. Cited in 2 reference(s)
  3. CD11c + resident macrophages drive hepatocyte death-triggered liver fibrosis in a murine model of nonalcoholic steatohepatitis Michiko Itoh, , Masato Tanaka, Yoshihiro Ogawa Published November 16, 2017 Citation Information: JCI Insight. 2017; 2(22) :e92902
  4. CD11c is a member of the leukocyte integrin family of adhesion proteins. It is reported to be expressed in normal tissues, mainly on myeloid cells, for example, in bone marrow myelocytes, premyelocytes, metamyelocytes, non-segmented and segmented neutrophils with high levels reported on tissue macrophages and monocytes and with lowest levels in.
  5. Search results for cd11c at Sigma-Aldrich. Summary: This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain

View mouse Itgax Chr7:128129547-128150657 with: phenotypes, sequences, polymorphisms, proteins, references, function, expressio Repetitive DE treatment for 3 weeks resulted in significant increases in CD11c + /CD11b + macrophages in whole lung-associated tissue. These cells displayed increased costimulatory molecule (CD80 and CD86) expression, enhanced phagocytic ability, and an increased production of IL-6, CXCL1, and CXCL2 Author Summary In the current study, we employed an experimentally induced type 2 diabetes mellitus (T2DM) model in wild type C57BL/6 mice and investigated the immune response to Mycobacterium tuberculosis (Mtb) infection. We found that natural killer (NK) and CD11c+ cell interactions in Mtb-infected T2DM mice led to increased IL-6 production, which drives the pathological immune response and.

Complement receptor type 4 (CR4)

CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580) CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7). The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9). Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9 CD11c + Gr-1 + cells can be separated into CD4 + and CD4 − populations. They express CD11b and MHC class II at very low levels and do not express CD40, CD80, CD86, or L-selectin. CD11c + Gr-1 + cells from BALB/c LNs display a similar pattern of surface marker expression (data not shown)

11.4.1.1.2 Dendritic cells

CD11c (also known as Leu-M5 or Integrin alpha X) [5D11] is a member of the leukointegrin family. CD11c is a cell surface adhesion receptor and is predominantly expressed in tissue macrophages, dendritic cells, monocytes, NK cells and granulocytes. CD11c has been shown to be both sensitive and specific for hairy cell leukemia (HCL CD11c: Products. CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the Integrin beta 2 subunit (CD18). This heterodimeric protein (Integrin alpha X beta 2) is also known as p150/p95 and complement receptor type 4 (CR4) CD11c is an adhesion receptor of the leukocyte function-associated family of molecules. This cell surface antigen is normally expressed on granulocytes, monocytes, natural killer cells, macrophages, and small populations of T- and B-lymphocytes

APC anti-mouse CD11c Antibody anti-CD11c - N41

Gr1+ CD11b+ myeloid cells promote metastasis (Cancer Cell 2008;13:23) Poor prognostic factor in preB acute lymphoblastic leukemia (Blood 2010;115:3763), cytogenetically unfavorable AML (Leuk Res 2013;37:122) Documents maturation of tumor cells in acute promyelocytic leukemia patients receiving all-trans retinoic acid therapy (cells become CD11b+, CD16+, Arch Pathol Lab Med 2003;127:e4) or. ① (P. 9) M1型+M2型 Iba1 M1型 CD11c M2型 CD163 or CD206 高 一般的に使用される組み合わせです。 ④ (P. 12) M1型+M2型 Iba1 M1型 CD197 M2型 CD163 or CD206 中 一般的に使用される組み合わせです。 ⑥ (P. 14) M1型+M2型 Iba1 M1型 CD38 M2型 Egr2 低 報告された精度は最も高いですが データ. Activated lymphocytes may express CD11c, in special conditions, like disease, because CD11c is not a mere cell marker, but it is an adhesion molecule (among other functions), and cells may.

CD11c. a marker of dendritic cells. PMID: 16142734. CD21. a reliable marker of follicular dendritic cells. PMID: 16280657. CD83. maps to chromosome 6p23. PMID: 10738529. specific for dendritic cells. PMID: 12452842. a marker of mature dendritic cells. PMID: 12113551. CD86. a specific marker for canine monocyte-derived dendritic cells 33 reviews. Compare Anti-CD11c Flow Cytometry Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more Dendritic cells (DC) and cytokines that expand myeloid progenitors are widely used to treat cancer. Here, we show that CD11c+DEC205+ DCs coexpressing α-smooth muscle actin and VE-cadherin home to perivascular areas in the ovarian cancer microenvironment and are required for the maintenance of tumor vasculature. Consequently, depletion of DCs in mice bearing established ovarian cancer by.

CD11c + MHCII lo GM-CSF-bone marrow-derived dendritic cells act as antigen donor cells and as antigen presenting cells in neoepitope-elicited tumor immunity against a mouse fibrosarcoma. Hakimeh Ebrahimi-Nik 1 Pancreatic ductal adenocarcinoma (PDAC) after complete surgical resection is often followed by distant metastatic relapse for reasons that remain unclear. In this study, we investigated how the immune response at secondary sites affects tumor spread in murine models of metastatic PDAC. Early metastases were associated with dense networks of CD11b+CD11c+MHC-II+CD24+CD64lowF4/80low dendritic. CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker ITGAX (Integrin Subunit Alpha X) is a Protein Coding gene. Diseases associated with ITGAX include Hairy Cell Leukemia and Benign Cephalic Histiocytosis.Among its related pathways are Innate Immune System and ERK Signaling.An important paralog of this gene is ITGAD

Video: CD11c Antibodies: Novus Biological

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Anti Mouse CD11c Antibody, clone N418 Bio-Ra

  1. David B. Lewis MD, in Hematology, Immunology and Infectious Disease: Neonatology Questions and Controversies (Second Edition), 2012
  2. Consequently, CD11c + myeloid blasts almost always co‐expressed CD11b whereas CD14 + cases of AML often comprised CD14 + CD11c + and CD14 + CD11c‐ subpopulations. It is concluded from these observations that CD11c immunophenotyping is a useful supplementary investigation, particularly in CD14‐ cases of myelomonocytic leukaemia
  3. ent marker protein in DCs, especially in vivo, remain poorly defined
  4. Copyright © 2020 Elsevier B.V. or its licensors or contributors. ScienceDirect ® is a registered trademark of Elsevier B.V.
  5. Lutz et al take issue with our recent article documenting the heterogeneity of CD11c+MHC II (major histocompatibility complex class II)+ cells in cultures of mouse bone marrow (BM) with GM-CSF (Helft et al., 2015). Their objections are grouped under sub-headings and are here addressed using the same structure

CR4 (CD11c /CD18 or p150,95), the third member of the leukocyte β 2-integrins, is closely related to CR3. The CR4 α chain (CD11c, 150 kDa) is ∼87% homologous in sequence to CD11b, and recognizes similar ligands including iC3b, fibrinogen and, according to some reports, even ICAM-1 Circulating CD11c+ DCs are typically CD11chigh and major histocompatibility complex (MHC) class IIhigh but lineage-negative (Lin–), i.e., lacking markers for other cell lineages, such as T cells, monocytes, B cells, NK cells, granulocytes, and erythroid cells. Human pDCs are CD11clowLin– and express high levels of CD123 (IL-3 receptor α-chain) and BDCA-2 (CD303 or CLEC4C), a type II transmembrane C-type lectin. The concentrations and surface phenotype of CD11c+ DCs in cord blood and adult peripheral blood are similar.3 An exception is CD86 (B7-2) T-cell co-stimulatory protein, which is lower on cord blood CD11c+ DCs. The concentration of pDCs in cord blood is significantly higher than in adult peripheral blood, and gradually declines after birth.3 Mutation details: For generation of CD11c.DTR BAC transgenic mice, a fusion construct composed of the DNAs for the human DTR, ovalbumin fragment containing sequences encoding amino acids 140-386, and eGFP was generated and inserted at the start codon of the CD11c gene in the BAC RPCI-24-7812. The eGFP component of the fusion protein failed to give a fluorescence signal, whereas the ovalbumin.

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Circulating neonatal DCs have selective limitations in the TLR-mediated upregulation of expression of molecules involved in T-cell co-stimulation by engagement of CD28 (CD80 and CD86) and other DC–T-cell interactions (MHC class II and CD40) compared to those of the adult.3 For example, cord blood CD11c+ DCs have reduced expression of CD40 in response to ligands for TLR-2/6 (Mycoplasma fermentans), TLR-3 (poly I:C), TLR-4 (lipopolysaccharide (LPS)), and TLR-7 (imiquimod), and reduced CD80 in response to TLR-3 or -4 ligands. In contrast, CD11c+ DC upregulation of CD40 in response to GU-rich single-stranded RNA, a TLR-8 ligand, is similar, as is MHC class II and CD86 upregulation after stimulation with TLR-3 and -4 ligands. Cord blood and adult peripheral blood pDCs also similarly upregulate HLA-DR, CD80, and CD86 after stimulation with TLR-9 ligands, i.e., synthetic DNA oligonucleotides lacking methylated CpG residues (CpG DNA).3CD11c+ DCs isolated from mouse mucosa such as Peyer's patches or respiratory tracts preferentially induce TH2 differentiation (Stumbles et al., 1998; Iwasaki and Kelsall, 1999; Khanna et al., 2000). By contrast, CD11c+ DCs isolated from mouse spleen preferentially induce TH1 differentiation. DCs derived from bone marrow precursors or from liver precursors also displayed different effector function in polarizing T helper cells. While liver-derived DCs produced high IL-10 and induced allogeneic T cells to undergo TH2 differentiation, bone marrow-derived DCs produced low IL-10 and induced allogeneic T cells to undergo TH1 differentiation during primary MLR in cultures or in allogeneic recipient mice after DC transfer (Khanna et al., 2000). The functional differences among different tissue DCs may result from differences in tissue cytokine microenvironments as well as in the lineage origin of different tissue DCs.Monocyte-derived dendritic cells (MDDCs) are produced by culturing cord blood or adult peripheral blood monocytes in vitro with cytokines, such as granulocyte–macrophage colony-stimulating factor, IL-4, and tumor necrosis factor (TNF)-α. In general, cord blood MDDCs have reduced levels of IL-12 p70 expression after stimulation, but it remains unclear if these and other functional and phenotypic differences apply to neonatal and adult DCs of the tissues. Gene expression profiling suggests that MDDCs may best model inflammatory DCs, which are generated from monocytes in vivo at sites of marked tissue inflammation, such as during Th1-driven reversal reactions of leprosy,3 but it is unclear to what extent inflammatory DCs are generated in the fetus and neonate.

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CST - CD11c (D1V9Y) Rabbit mA

Anti-CD11c antibodies can be readily obtained from commercial sources. The CD11c protein is a reported synonym for the human gene ITGAX, encoding integrin subunit alpha X. The full-length protein is 127,829 daltons in mass and has been noted to be membrane-associated. This protein is reported to contain sites of glycosylation F, Hairy cell leukemia (HCL) case with uniform bright CD123. Analysis gate was drawn around the bright CD20+ and CD11c+ HCL cells. G, HCL-variant (HCLv) case with dim CD123. Analysis gate was drawn around the bright CD20+ and CD11c+ HCLv cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells. It is upregulated on IEL and lymph-node T cells following in vivo activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b

E Flow Cytometric Gating Strategies

Plasmid ANTI-CD11c IMMUNOTOXIN from Dr. Jose Conejo-Garcia's lab contains the insert ANTI-CD11C IMMUNOTOXIN and is published in Cancer Res. 2008 Sep 15. 68(18):7684-91. This plasmid is available through Addgene RESULTS CD11c − MHC2 low Adipose Tissue Macrophages Are a Novel and Dynamic Subpopulation of Macrophages in the Adipose Tissue of LDLR −/− Mice. vAT isolated from LDLR −/− mice receiving a HFD for 16 weeks was prepared for flow cytometry and stromal vascular cells were assessed for F4/80, the classical murine macrophage marker and CD11c, which is discriminative for M1 ATMs CD11c is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells. It associates with CD18 to form the CD11c/CD18 complex that binds fibrinogen and has been reported to be a receptor for iC3b and ICAM-1

ITGAX Gene - GeneCards ITAX Protein ITAX Antibod

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some. ITGAX (CD11c) is the integrin alpha X chain protein that functions as a fibrinogen receptor. ITGAX plays a role in monocyte adhesion and chemotaxis, and can induce cellular activation and trigger neutrophil respiratory burst. In cancer, it is positive in leukemia, including the hairy cell, acute nonlymphocytic and B-cell chronic lymphocytic.

Critical role of integrin CD11c in splenic - PNA

CD11c antibody LS-C112587 is a PerCP-conjugated mouse monoclonal antibody to CD11c (ITGAX) from human. It is reactive with human and monkey. Validated for Flow, IHC and IP CD11c transgene identifies a discrete network of dendritic cells in mouse embryonic central nervous system and in postnatal brain cells. The CD11c antibody was both sensitive and specific for hairy cell leukemia, even in cases with minimal infiltration Download as PDFSet alertAbout this pageImmunohistology of Non-Hodgkin LymphomaAlvin W. Martin, in Diagnostic Immunohistochemistry (Third Edition), 2011 However, CD11c+ was significantly higher in both adhered and suspended cell population by culture method but kit method gave more CD11c+ from suspended cells population only. On the other hand, using both methods, immature DC expressed moderate level of MHC class II molecules as well as low levels of CD40 and CD86

CD11b definition of CD11b by Medical dictionar

A unique subset of T-bet-expressing B cells accumulates with aging and in autoimmunity. Pernis and colleagues show that dysregulation of the transcription factor IRF5 occurs after loss of the Rho. The CD11c antigen (integrin alphaX subunit / leucocyte surface antigen p150) is a member of the integrin family. Like other leucocyte integrins (CD11a, CD11b, CD11d), CD11c is non-covalently associated with the integrin beta2 subunit (CD18) (6, 7). CD11c is expressed mainly by monocytes, macrophages and NK (Natural Killer) cells, an

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